FIG. 5.
Kinetics of levansucrase (A) or α-amylase (B) processing and release by strains NIG1156 and QB112 or GM96104 and GM96101, respectively. Four generations after induction of the strains with sucrose (8 mM for NIG1156 and QB112 or 60 mM for GM96104 and GM96101), cultures were shifted to 37°C (A) or 42°C (B) for 8 min. Samples (2 ml) were labelled with 0.15 mCi of [35S]methionine for 45 s and chased with an excess of nonradioactive methionine. Cell extracts were immunoprecipitated, and supernatants were dialyzed, lyophilized, and finally analyzed by SDS-PAGE.