Fig. 1.

Schematic presentation of PIN particle dirRT-qPCR of viral RNA from saliva samples. The sample is heat-treated to release viral RNA for RT, amplification, and detection inside PIN particles. Following viral lysis, the mixture is mixed with one-step RT-qPCR reagents and loaded into a microfluidic qPCR chip containing PIN particles. The target viral RNAs are captured and selectively reverse-transcribed using immobilized acrydite forward primer, followed by 40 cycles of qPCR using PCR primer (reverse primer) and detection in real-time