TABLE 1.

Primer names and sequences.

Primer name	Amplification efficiency (%)	Sequence (5′to 3′)	Amplicon sizeL (bp)
qPCR -FST F	92.3	ACT​CGG​ACT​ACA​CGG​CCT​AT	77
qPCR -FST R	92.3	AGA​ACT​GTC​CCC​GTA​TTG​CG	77
qPCR -MSTN F	94.9	CGG​ACA​AGA​TGC​CTG​TGA​GT	88
qPCR -MSTN R	94.9	TGT​GTG​TCC​TGT​TCA​CCG​AG	88
qPCR- APOA1 F	93.5	GGA​TCT​GCG​CAC​CTC​TAT​CC	101
qPCR- APOA1 R	93.5	GGA​TCT​GCG​CAC​CTC​TAT​CC	101
qPCR-ghra F	94.1	AGC​CAG​AGC​GTA​GCA​AAC​TT	156
qPCR-ghra R	94.1	GTT​GGG​GGT​GAG​TAA​GAG​GC	156
qPCR-AKT F	93.5	CCG​AGA​TTG​TCT​CCG​CTC​TC	170
qPCR-AKT R	93.5	GGA​CAC​CAC​TTG​GTC​TCT​CG	170
qPCR-Keap1 F	94.2	CGT​GGG​TGT​AGC​CAT​TAC​CA	144
qPCR-Keap1R	94.2	TGA​CTG​TGC​TGC​TGA​CTC​TG	144
qPCR-Nrf2 F	92.6	GCC​AAT​CAC​TAA​TGC​GGC​AG	80
qPCR-Nrf2 R	92.6	GCC​ACT​GTT​GTA​GCC​ACT​CT	80
qPCR-Myf5 F	92.7	AGG​GAC​TCC​TCT​CGT​GCA​TT	146
qPCR-Myf5 R	92.7	CTC​CAT​GCC​AGG​ACC​AAA​GT	146
qPCR-MyoD1 F	92.3	ACG​CCA​TCA​GCT​ACA​TCG​AG	157
qPCR-MyoD1 R	92.3	GTA​ACA​GGT​GTC​CGC​TCA​CT	157
qPCR-MyoG F	95.1	GGT​GTC​CTC​CCT​AAA​CCA​GC	130
qPCR-MyoG R	95.1	CCG​AAC​TAG​GCT​CAC​TCG​AC	130
qPCR-TGF-β1 F	95.0	GTG​GGG​AAA​TCT​GCC​CGT​TA	147
qPCR-TGF-β1 R	95.0	CAG​CCG​AAG​TTG​GAA​GAC​CT	147
Beta-actin F	92.2	CTC​TTC​CAG​CCA​TCC​TTC​CT	220
Beta-actin R	92.2	TCA​GGT​GGG​GCA​ATG​ATC​TT	220
5′RACE-FST F1	—	TTGCCAGCTTGAACTT	-
5′RACE-FST F2	—	TGATGTTCCATAAGGTGA	—
5′RACE-FST F3	—	AATGCCCGGGTGGAGGTG	—
3′RACE-FST R1	—	GTC​CAG​AGA​GCC​GAA​CAG​ATG​AGG	—
3′RACE-FST R2	—	GCG​TGT​TCA​ATG​GGA​GTT​CTG​CTG	—
5′RACE-MSTN F1	—	GCAGGAACACCGTGGT	—
5′RACE-MSTN F2	—	GCAGGTGAACCCACAGCT	—
5′RACE-MSTN F3	—	TTGACTCGGCTGGAACTT	—
3′RACE-MSTN R1	—	AGC​GTT​ACA​AGG​CCA​ACT​ACT​GCT	—
3′RACE-MSTN R2	—	TGC​AGA​AGT​ACC​CAC​ACA​CTC​ACC	—