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. 2022 Jul 25;16:831803. doi: 10.3389/fncel.2022.831803

FIGURE 1.

FIGURE 1

Extracellular recordings of CIII neurons in a Drosophila larval filet. (A,B) The larva filet was placed in an experimental chamber with running HL3 saline. To produce a controlled temperature decrease during the stimulus, the superfusion path was switched to the one that goes through the chiller, and chilled saline was delivered. The saline temperature was constantly monitored by a thermometer probe. The saline was grounded with an Ag-AgCl wire in an agar bridge. We recorded spiking activities from two subtypes of CIII neurons, ddaA or ddaF, located in the dorsal cluster of sensory neurons. To do this, the cell body with a portion of its neurite was gently sucked up into the pipette. (C) Image of CIII neurons (ddaA and ddaF) labeled by GAL419–12, UAS-mCD8::GFP, with the electrode (a dotted line) attached to ddaA.