TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| E. coli strains | ||
| W3110 | IN(rrnD-rrnE) rph-1 | K. Mizobuchi |
| TG1 | supE hsdΔ5 thi Δ(lac-proAB) F’ traD36 proAB+ lacIqlacZΔM15 | 15 |
| MC1000 | araD139 Δ(ara-leu)7697 ΔlacX74 galU galK rpsL | 5 |
| BL21 (DE3) | F′ dcm ompT hsdS(rB− mB−) gal λDE3(lacI lacUV5-T7 gene 1 ind-1 Sam7 nin-5) | RIKEN DNA Bank |
| UM122 | rpoS::Tn10 | A. Ishihama |
| YU449 | W3110 ssnA::kan | This work |
| YU450 | W3110 rpoS::Tn10 | This work |
| YU451 | W3110 ssnA::kan rpoS::Tn10 | This work |
| YU381 | MC1000 rpoS::Tn10 | 21 |
| Plasmids | ||
| pBR322 | Ampr Tetr | 3 |
| pACYC177 | Ampr Kanr | 6 |
| pBRSSNA | pBR322 with the 2.9-kb PstI fragment bearing ssnA | This work |
| pBRSSNA-ND | pBR322 with the 2.3-kb PstI-NruI fragment from pBRSSNA | This work |
| pBRSSNA-NP | pBR322 with the 0.6-kb PstI-NruI fragment from pBRSSNA | This work |
| pBRSSNA-MD | pBRSSNA lacking the 0.5-kb MluI fragment | This work |
| pACYCSSNA | pACYC177 with the 2.9-kb PstI fragment bearing ssnA | This work |
| pVEX11 | Ampr, pBR322 with T7 promoter of φ 10 gene and its terminator | 20 |
| pVEXSSNA | pVEX11 with the 1.3-kb XbaI-BamHI fragment bearing ssnA | This work |
| pJQ200mp18 | sacB Genr | 14 |
| pJQ200SSNA | pJQ200mp18 with the 2.9-kb PstI fragment bearing ssnA | This work |
| pJQ200SSNA-K | pJQ200SSNA derivative with insertion of kan at the NruI site | This work |
| pCB182 | lacZ galK Ampr | 17 |
| pSSNALAC | ssnA-lacZ operon fusion in pCB182 | This work |