Fig. 2. Vaccination of Syrian hamsters with AZD2816 or AZD1222 followed by AZD2816 reduces lower respiratory tract infection by the Beta VoC.

Twenty-eight days post final vaccination, hamsters were challenged with 10⁴ TCID₅₀ of the Beta variant, via the intranasal route. A Relative weight in comparison to day 0. Thick lines = median weight per group, thin lines = individual animals, dotted line = 100% relative weight. Days 0–3 N = 18, Days 4–5, N = 12, Days 6–14 N = 6. B Boxplot (minimum to maximum) of relative weight at day 7. Statistical significance was determined via a Kruskall-Wallis test followed by Dunn’s multiple comparisons test. C Boxplot (minimum to maximum) of subgenomic SARS-CoV-2 RNA (sgRNA) (left panel) and infectious virus isolation (right panel) in lung tissue harvested on day 3 and 5 (N = 6). Statistical significance was determined via a Kruskall Wallis test followed by Dunn’s multiple comparisons test. Dotted line = limit of detection. D Boxplot (minimum to maximum) of sgRNA (left panel) and infectious virus (right panel) in oropharyngeal swabs taken on day 1 (sgRNA only, N = 18), 3 (N = 18), 5 (N = 12), and 7 (sgRNA only, N = 6). Statistical significance was determined via a mixed-effects analysis followed by Dunnett’s multiple comparisons test, comparing vaccinated groups against control group. Dotted line = limit of detection. E Boxplot (minimum to maximum) of the area under the curve (AUC) analysis of shedding as measured by sgRNA analysis in swabs collected on 1–7 days post-inoculation. Statistical significance was determined via a Kruskall-Wallis test followed by Dunn’s multiple comparisons test. N = 6, circles = hamsters vaccinated with AZD2816, squares = hamsters vaccinated with AZD1222 followed by AZD2816, triangles = hamsters vaccinated with ChAdOx1 GFP. All boxplots are drawn from first quartile to third quartile, with a line at the median. Whiskers go from each quartile to minimum or maximum values. Source data are provided as a Source Data file.