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. 2022 Aug 8;14(8):e16423. doi: 10.15252/emmm.202216423

Figure 1. Validation of the HCM and HCMrep hiPSC lines.

Figure 1

Sequencing of the HCM and HCMrep hiPSC clones. The HCMrep clone revealed on the +strand the repaired mutation (T>C (repair), violet), the 2 silent mutations from the repair template (C>A and G>C, red), and the on‐target defect (T>C (on‐target), blue). Note that the SnapGene software sometimes underlined the presence of a mutation with a red square at the nucleotide site, but sometimes did not.