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. 2022 Jul 13;14(8):e14759. doi: 10.15252/emmm.202114759

Figure 1. Graft‐derived macrophages in demyelinating lesions.

Figure 1

  • A
    Bone marrow isolated from actin‐GFP mice was transplanted into preconditioned recipients.
  • B
    Flow cytometry analyses of recipient blood 2 months post‐transplant. (Bi) Transplanted cells detected by GFP fluorescence. (Bii) CD11b labeling shows a large proportion of monocytes expressing GFP.
  • C
    Demyelinating lesions in chimeric mice at 3 dpl. (Ci) Demyelination was induced by injecting LPC in the spinal cord white matter. (Cii–Civ) Co‐immunolabeling for GFP, MBP, and a mixture of CD11b/CD68, showing graft‐derived macrophages in the lesion (identified by lack of MBP staining). White lines indicate lesion borders. Scale bar 20 μm. MBP—myelin basic protein; GFP—green fluorescent protein.

Source data are available online for this figure.