Figure 7. Oligodendrogenesis and remyelination are accelerated in Sema3F chimeras.

• A, B
  Labeling for APC/CC1, a marker of oligodendrocytes. Dotted lines indicate the lesion border. APC⁺ cells are scarce in the lesions of GFP mice (A). B. APC⁺ cells in the lesion of Sema3F mice.
• C
  Quantification of APC⁺ cells (Mann–Whitney test; P = 0.0061 for 7 dpl, P = 0.0081 for 10 dpl; n = 6–7 mice/group). *P < 0.01.
• D, E
  EM images of the lesion in GFP (D) and Sema3F (E) chimeras at 14 dpl. Thin myelin sheaths in Sema3F mice (yellow arrows).
• F
  Higher power image of a new myelin sheath in a Sema3F mouse. Fi. Inset of yellow square in (F).
• G
  Quantification of remyelinated axons (Mann–Whitney test: P = 0.03, n = 6 mice/group). *P < 0.05.
• H
  In Sema3F mice, oligodendroglial cells (O) and early remyelination (*) are frequently observed in macrophage (M) vicinity. Hi. Inset of yellow dashed square in (H).

Data information: Scale bars 20 μm (A, B), 1 μm (D, E), and 500 nm (F).

Source data are available online for this figure.