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. 2022 Jul 4;14(8):e15386. doi: 10.15252/emmm.202115386

Figure 2. CD IgA1 N and O‐glycosylations do not recapitulate conventional IgA glycosylation patterns.

Figure 2

  • A
    Full MS spectrum of glycopeptides released from CD IgA1 trypsin digest. Main N340‐glycoforms of the IgA1 glycopeptide [332–353] (LAGKPTHVNVSVVMAEVDGTCY) are annotated using CFG nomenclature. Appendix Table S4 summarizes a list of N‐glycopeptides identified. # is the peptide [43–76] of immunoglobulin kappa constant chain (UniProtKB P01834). *Isobaric structures not differentiated by MS/MS experiments (not exhaustive N‐glycans illustrations). (HYTNPSQDVTVPCPVPSTPPTPSPSTPPTPSPSCCHPR).
  • B
    O‐glycoforms identified for CD.
  • C
    O‐glycoforms identified for UC.

Data information: *corresponds to IgA1 peptide [264–273] (WLQGSQELPR). #shows contamination by other multiply charge species covering the glycoforms signals. Details related to O‐glycoforms are given in Appendix Table S7. CD: n = 3; UC: n = 1 (biological replicates). All patient samples have been tested in technical duplicates.