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. 2022 Aug 3;83:104200. doi: 10.1016/j.ebiom.2022.104200

Figure 4.

Figure 4

EGFR-19Del/T790M/L792F drove the infiltration of M2-like macrophages. (a-b). Representative images (a) and histogram (b, p=0.022 or 0.046, respectively. Statistical differences were evaluated using t test) show migrated cells of THP-1 cultivated in conditional medium derived from H1975 control, H1975 L792Fcis, PC-9 control, and PC-9 L792Fcis cells by Boyden chamber assay (n=3). The scale bar in (a) was 100 μm. (c). qRT-PCR analyses of CD206, IL-10, and ARG1 expression in the THP-1 cultivated in conditional medium from H1975 control and H1975 L792Fcis cells (p=0.009, 0.0004 or 0.011, respectively. Statistical differences were evaluated using t test). (d). The protein level of CD206, IL-10, and ARG1 by western blot assay. (e-f). qRT-PCR analyses of CD206, IL-10, and ARG1 expression in the formed tumors of nude mice derived from H1975 control, H1975 L792Fcis(e, p=0.00005, 0.004 or 0.004, respectively), PC-9 control and PC-9 L792Fcis(f, p=0.016, 0.019 or 0.011, respectively) (both n=3, Statistical differences were evaluated using t test). Data are presented as the means ± standard deviation.