TABLE 1.
E. coli strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| E. coli | ||
| LE392 | F−hsdR514 (rK− mK−) lacY1 supE44 supF58 galK2 galT22 trpR55 metB1 | |
| ISM612 | [leu(UGA) lacZ659(UGA) trpA9605(UAG) his29(UAG) ilv thyA metB argH rpoB rpsL prfB3] pISM3001 | 21, 36 |
| ISM614 | LE392 [F−hsdR514 (rK− mK−) lacY1 supE44 supF58 galK2 galT22 trpR55 metB1] pISM3001 | This study |
| ISM647 | XL1-Blue [recA1 lac endA1 gyrA46 thi hsdR17 supE44 relA1 (F′ proAB+ lacIqlacZΔM15 Tn10)] pISM3001 | This study |
| DPWC | F+ mating donor | Gold Biotechnologies |
| BW26 | F− mating recipient, Kanr | Gold Biotechnologies |
| CSR 603 | (recA1 uvrA6 phr-1) maxicell strain | 33 |
| Plasmids | ||
| pISM3001 | trpT gene cloned behind a lac promoter in the vector pACYC184, Cmr | 36 |
| pISM4170 | 6.6-kb SacI fragment from phage 13-2B cloned onto pMOB, Apr | This study |
| pISM4172 | 7.0-kb SacI fragment from phage 13-2B cloned onto pMOB, Apr | This study |
| pISM4175 | 2.2-kb ClaI fragment from pISM4172 cloned into pMOB, Apr | This study |
| pISM4176 | 2.6-kb ClaI fragment from pISM4172 cloned into pMOB, Apr | This study |
| pMOB | Cloning vector for Tn1000 mutagenesis, Apr | Gold Biotechnologies |