FIG. 1.

Analysis of PTA from T. maritima by SDS-PAGE at various steps of the purification procedure. Fractions with the highest specific activities obtained after various chromatographic steps (see Materials and Methods) were used. Protein was denatured in SDS and separated in 14% polyacrylamide slab gels (8 by 7 cm) (19), which were stained with Coomassie brilliant blue R 250. Lanes: 1 and 8, molecular mass standards (Sigma); 2, 100,000 × g supernatant, 12 μg of protein; 3, DEAE-Sepharose, 10 μg of protein; 4, Q-Sepharose, 10 μg of protein; 5, phenyl-Sepharose, 8 μg of protein; 6, Superdex 200, 6 μg of protein; 7 Mono Q, 2 μg of protein. The positions of molecular mass standards are indicated on the left.