TABLE 1.

Molecular and kinetic properties of purified PTA from eubacteria and M. thermophila

Organism	Molecular massa (kDa)		Apparent Kmb (mM)				Reference(s)
	Native enzyme	Subunit	Ac-CoA	Ac-P	Pi	CoA
Thermotoga maritima	170 (α4)	34	0.023	0.024	0.11	0.03	This work
Methanosarcina thermophila	52 (α)	43	—	0.17	—	0.09	21
Clostridium thermoaceticum	88 (α4)	20	—	—	—	—	11
Clostridium acidiurici	63–75	—	—	—	—	—	31
Clostridium kluyveri	38–41	—	0.79–1.2	0.78	17	0.12	3, 18
Bacillus subtilis	90	—	0.06	0.48	10	0.096	28
Escherichia coli	160–250 (α2)	81	—	3	—	0.32	37, 46
Lactobacillus fermenti	68	—	—	—	—	0.087	26
Rhodopseudomonas palustris	55 (α)	52.5	—	4.7	—	0.15	42
Veillonella alcalescens	75–85 (α2)	32–40	—	195	—	—	45

^aMolecular masses of native enzymes were determined by gel filtration, and those of subunits were determined by SDS-PAGE. The proposed subunit composition of the native enzyme is given in parentheses. 

^bAc, acetyl; P, phosphate; P_i, inorganic phosphate; —, not determined.