Figure 5.

DE and DAS analyses of pakchoi response to heat stress. (A) Flow chart showing the distribution of the 17 771 DE and 650 DAS genes. The DE and DAS gene sets differ markedly, with only 432 genes in common. (B) Classification of gene expression heat map of responsive HSPs under heat stress. (C) Distribution of the responsive AS events that generate nonsense-mediated mRNA decay (NMD) in two cultivars. (D) Exon–intron structure of representative transcripts of four AS genes. (E) The colored bar graphs represent the inclusion/exclusion ratio of the IR events of DAS genes as identified from RNA-seq and RT–qPCR. (F) Model of the heat-responsive pathway and genome-wide transcriptional and post-transcriptional regulation.