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. 2022 Aug 8;12(8):e12177. doi: 10.1002/clt2.12177

FIGURE 2.

FIGURE 2

Immunological characterization of rPru du 1. (A) Specific IgE to almond extract and rPru du 1 of sera from 17 almond allergic patients and correlation between sIgE levels to rPru du 1, almond extract and rBet v 1 in almond allergic patients (Spearman correlation). (B) IgE inhibition ELISA. Sera were inhibited with increasing concentrations of rBet v 1 (0.0001–10 μg/ml) and rPru du 1 (0.001–100 μg/ml). Horizontal bars indicate medians ± interquartile ranges. Inhibitions with other allergens were performed at 10 μg/ml. *Inh. conc.: Inhibitor concentration. (C) Basophil activation assay with RS‐ATL8 rat basophil leukemia (RBL) cells sensitized with sera from 14 patients (Table S1)