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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Biomater Adv. 2022 Feb 14;134:112712. doi: 10.1016/j.msec.2022.112712

Figure 4. mMSC solid spheroid formation within macroporous hydrogels.

Figure 4.

(A) Live/Dead staining of mMSCs immediately after microencapsulation into PEGNB-Dopa microgels. mMSCs with a density of 2×106 cells/mL, 1×107 cells/mL, and 2×107 cells/mL in OptiPrep were used for encapsulation. (B) Live/Dead and cytoskeleton fluorescence staining of mMSC spheroids after 7 days of culture. Scale bar = 100 μm. (C) Quantification of the sizes of microgels, cavities, and mMSC spheroids formed with various cell loading concentrations after 7 days of culture.