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. Author manuscript; available in PMC: 2022 Aug 9.
Published in final edited form as: Mol Microbiol. 2020 Oct 16;115(2):290–304. doi: 10.1111/mmi.14616

FIGURE 5.

FIGURE 5

Purified Fpn releases two fimbrial proteins from the cell surface. Wild-type (WT) and isogenic fpn− ETBF strains transformed with a derivative of pFD340 that encodes a FLAG-tagged version of Mfa1 (BF9343_4231), Mfa2 (BF9343_3703) or a NigD-like protein (BF9343_2956) were grown to mid-log phase. In (a) cells were separated from the culture medium by centrifugation. In (b) intact cells were pelleted and mock treated or treated with PK for 30 min at 37°C. In (c) intact fpn− cells were mock treated or treated with purified Fpn or the Fpn (C180A) mutant for 30 min at 37°C. Whole cell (WC) and culture medium (sup) fractions were then analyzed by Western blot using anti-FLAG-tag and anti-SurA antisera