Figure 1.

Development of murine model of pseudo-allergic reaction in the oral cavity. (A, B) Chloroacetate esterase staining or toluidine blue staining of serial tissue sections in the inside of the lower lip from Balb/c mice (n = 3) under steady conditions. (A) Chloroacetate esterase-stained (left panel) or toluidine blue-stained (right panel) mast cells are seen. (B) The percentage of both chloroacetate esterase- and toluidine blue-stained mast cells among chloroacetate esterase-stained mast cells. The total count was 59, 33, or 45 mast cells in serial tissue sections from three mice. Data are representative of two independent experiments. (C) Surface expression levels of FcεRIα and c-Kit among CD45⁺ hematopoietic cells in the inside tissue of the lower lip from Balb/c mice under steady conditions. FcεRIα⁺c-Kit⁺ mast cell populations (1.17%) among CD45⁺ cells (100%) were seen. Data are representative of three independent experiments. (D) A schematic representation of murine models of compound 48/80-induced pseudo-allergic reaction in oral cavities. (E) Injection of compound 48/80 inside the lower lip caused dye extravasation in the skin from WT mice but not from Kit^W-sh/^W-sh mice. Representative pictures of the neck skin from WT mice injected with compound 48/80 (left/lower) or PBS (left/upper) and from Kit^W-sh/^W-sh mice injected with compound 48/80 (right/lower) or PBS (right/upper) (F) Quantification of the Evans blue dye that extravasated into the neck skin from WT or Kit^W-sh/^W-sh mice injected with compound 48/80 or PBS. n =4 per group; data are presented as mean ± SD. Data are representative of two independent experiments. Brown-Forsythe and Welch ANOVA with Dunnett T3 multiple comparisons. **P < 0.01.