Figure 2.

Development of murine model of IgE- and mast cell-mediated allergic reaction in oral cavities. (A) A schematic representation of murine models of OVA-induced, IgE- and mast cell-dependent allergic reaction in oral cavities. (B, C) Serum levels of total IgE (B) and OVA-specific IgE (C) from WT mice (n = 8) or Kit^W-sh/^W-sh mice (n = 10) intraperitoneally injected with OVA plus alum twice at a 2-week interval or from non-sensitized WT mice (n = 8) or Kit^W-sh/^W-sh mice (n = 8). (D) Quantification of the Evans blue dye that extravasated into the neck skin from OVA-sensitized WT mice stimulated by OVA (n = 4) or PBS (n = 4) or Kit^W-sh/^W-sh mice stimulated by OVA (n = 6) or PBS (n = 4). Data are presented as mean ± SD. Data are representative of two independent experiments. Brown-Forsythe and Welch ANOVA with Dunnett T3 multiple comparisons. *P < 0.05.