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. 2022 Jul 25;13:935480. doi: 10.3389/fimmu.2022.935480

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Copyright © 2022 Chamtim, Suwan, Dong, Sirisuay, Areechon, Wangkahart, Hirono, Mavichak and Unajak

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression and purification of Tis9 and Tis10 proteins. (A) Western blot analysis of both proteins from the E. coli expression system. Tis9 and Tis10 proteins expressed approximately 32 kDa and 37 kDa, respectively (Lane M: prestained protein marker). (B, C) SDS-PAGE analysis of (B) Tis9 and (C) Tis10 proteins purification with coomassie blue staining. (Lane M: prestained protein marker; Lane S: supernatant; Lane P: precipitation of the E. coli was induced; Lane F: unbound flowthrough; Lane W: wash fraction; Lanes: E1-E2 = elute fractions.