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. 2022 Jul 25;13:935480. doi: 10.3389/fimmu.2022.935480

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Copyright © 2022 Chamtim, Suwan, Dong, Sirisuay, Areechon, Wangkahart, Hirono, Mavichak and Unajak

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cumulative mortality curves of the recombinant protein vaccines (Tis9, Tis10 and Tis9+Tis10 groups) and DNA vaccines (pcDNA-Tis9, pcDNA-Tis10 and pcDNA-Tis9+Tis10 groups) after challenge with 1 x 10⁵ TCID50 of TiLV. Differences among the groups were analyzed using one-way ANOVA and Tukey’s post hoc tests. Statistical significance is indicated by single asterisk (*, P < 0.05) or double asterisks (**, P < 0.01) or ns (not significant) compared to the PBS or pcDNA3.1 (negative control) groups at the termination day (day 28).