Table 1.
ORF-encoded protein prediction and biochemical properties of TiLV segments 1-10 (Tis1 - Tis10) proteins.
| TiLV genesegment | GenBank accession no. a | Gene segment length (bp) | Predicted protein name | Predicted protein length (aa) | MW (kDa) | pI | Hydropathicity | Aliphatic index |
|---|---|---|---|---|---|---|---|---|
| Segment 1 | OL469273 | 1560 | Tis1 | 519 | 57.109 | 8.36 | -0.150 | 84.95 |
| Segment 2 b | OL469274 | 840 | Tis2 | 279 | 31.492 | 9.39 | -0.451 | 76.95 |
| Segment 3 | OL469275 | 1260 | Tis3 | 419 | 47.689 | 7.56 | -0.422 | 81.24 |
| Segment 4 | OL469276 | 1076 | Tis4 | 356 | 38.577 | 9.13 | -0.140 | 85.81 |
| Segment 5 | OL469277 | 1032 | Tis5 | 343 | 38.05 | 8.59 | 0.262 | 101.69 |
| Segment 6 | OL469278 | 936 | Tis6 | 311 | 35.68 | 8.79 | -0.316 | 79.26 |
| Segment 7 | OL469279 | 588 | Tis7 | 195 | 21.853 | 9.67 | -0.310 | 76.97 |
| Segment 8 | OL469280 | 525 | Tis8 | 174 | 19.536 | 9.27 | -0.167 | 100.34 |
| Segment 9 | OL469281 | 360 | Tis9 | 119 | 13.539 | 6.04 | -0.143 | 82.61 |
| Segment 10 | OL469282 | 342 | Tis10 | 113 | 12.803 | 4.63 | -1.241 | 50.09 |
a
Unpublished data (accessed on 25 December 2021).
b
The PCR product of TiLV segment 2 was amplified with primers Tis-2-F and Tis-2-R ( Supplementary Table 1 ) to obtain the Tis-2 protein, which had 279 amino acids and was translated from the second start codon (position 184).