Table 3.
Cumulative mortality and RPS values of vaccinated fish at 28 days post TiLV challenge.
| Group | Dose of vaccine (µg/fish) | Administration route | Number of fish challenged | Challenge dose (TCID50/fish) | No. of dead fish/total fish | Cumulative mortality (%) | RPS (%) |
|---|---|---|---|---|---|---|---|
| PBS | – | IP | 10 (3 replicates) | 1 × 105 | 18/30 | 60.00 ± 10.00 | – |
| pcDNA3.1 | – | IM | 10 (3 replicates) | 1 × 105 | 15/30 | 50.00 ± 00.00 | 16.67 ± 00.00a |
| Tis9 | 200 µg | IP | 10 (3 replicates) | 1 × 105 | 13/30 | 43.33 ± 5.77 | 27.78 ± 9.62a |
| Tis10 | 200 µg | IP | 10 (3 replicates) | 1 × 105 | 10/30 | 33.33 ± 15.28 | 44.45 ± 25.46ab |
| Tis9 + Tis10 | 200 µg | IP | 10 (3 replicates) | 1 × 105 | 8/30 | 26.67 ± 5.77 | 55.56 ± 9.62b |
| pcDNA-Tis9 | 5 µg | IM | 10 (3 replicates) | 1 × 105 | 11/30 | 36.67 ± 5.77 | 38.89 ± 9.62ab |
| pcDNA-Tis10 | 5 µg | IM | 10 (3 replicates) | 1 × 105 | 9/30 | 30.00 ± 10.00 | 50.00 ± 16.67b |
| pcDNA-Tis9 + pcDNA-Tis10 | 5 µg | IM | 10 (3 replicates) | 1 × 105 | 7/30 | 23.33 ± 5.77 | 61.11 ± 9.62b |
Data are represented as means ± SDs (n = 3). Differences in RPS were analyzed using one-way ANOVA and Tukey’s post hoc tests, compared with the pcDNA3.1 (control group). Different letters indicate significant differences (P < 0.05). IP, intraperitoneal injection; IM, intramuscular injection.