Table 3. Library quantification and sequencing results; values shown are for both shearing methods (mechanical; enzymatic).

Species	Input DNA for library preparation (ng)	Average library size (bp)	Final library concentration (ng/µL)	Total raw reads	Calculated genome coverage	Reads mapped to mitogenome	Percent reads mapped	Avg. mitogenome coverage
Gymnura altavela	170	318; 326	2.50; 1.98	2,193,690; 2,224,022	0.18; 0.19	201; 1,141	0.01; 0.05	1.6; 8.9
Gymnothorax fimbriatus	78	353; 370	0.498; 1.31	1,522,912; 1,809,632	0.10; 0.12	2,336; 2,647	0.15; 0.15	20.7; 23.0
Gymnothorax undulatus	51	356; 379	0.984; 2.82	2,146,906; 5,168,856	0.14; 0.34	984; 2,245	0.05; 0.04	8.7; 19.5
Saurida nebulosa	27.6	353; 391	0.382; 1.87	2,120,606; 3,174,282	0.21; 0.31	5,290; 5,603	0.25; 0.18	47.1; 48.7
Tylosurus crocodilus	4.6	380; 390	0.156; 0.27	463,424; 2,451,640	0.07; 0.37	1,065; 5,507	0.23; 0.22	9.4; 48.6
Myripristis vittata	25.1	337; 354	0.352; 1.42	1,290,468; 2,342,102	0.21; 0.39	754; 1,615	0.06; 0.07	6.7; 13.8
Neoniphon sammara	17.1	352; 375	0.286; 0.876	2,276,566; 4,265,046	0.43; 0.80	2,169; 3,957	0.10; 0.09	19.3; 34.6
Brosme brosme	41	334; 392	0.366; 1.79	1,027,598; 1,635,836	0.37; 0.69	3,321; 5,148	0.32; 0.31	29.4; 45.1
Scomberoides lysan	33.9	340; 378	0.344; 1.30	2,621,818; 4,818,598	0.54; 0.99	7,249; 12,324	0.28; 0.26	64.2; 107.9
Forcipiger flavissimus	109	351; 378	1.06; 2.96	1,993,702; 2,116,356	0.41; 0.44	1,193; 1,311	0.06; 0.06	10.5; 11.1
Ostracion whitleyi	86.5	340; 371	1.32; 3.34	2,054,668; 2,473,712	0.31; 0.38	2,369; 3,069	0.12; 0.12	20.596; 27.089
Canthigaster amboinensis	19.1	331; 371	0.224; 0.678	1,880,384; 2,868,978	0.68; 1.04	6,070; 8,672	0.32; 0.30	53.132; 76.469