Figure 7.

YQCM decoction exhibits anti-apoptotic activity, protecting Hei-oc1 cells from DNA damage induced by H₂O₂. (A) HEI-OC1 cells were incubated with YQCM (0, 50, 75, 100, 125, 250, and 500 μg/mL) for 24 h, and then cell viability was determined by CCK8 assay. (B) Cells were exposed to 100 μM H₂O₂, and cell viability with and without YQCM pre-treatment under oxidative conditions was determined by CCK-8 assay as well. (C and D) Cells were pre-treated with YQCM (10, 30, 90 μg/mL) for 24 h, and the mitochondrial membrane potential of cells with and without YQCM retreatment under oxidative conditions were examined by TMRE immunofluorescence. The fluorescence intensity was quantified with the High-content imaging system (n = 3). (E–G) Western blotting of DNA-damage marker, γH2AX, and apoptosis protein, cleaved caspase-3 (n = 3). The values represent the means ± SD, *p < 0.05 and **p < 0.01 vs. control, #p < 0.05 and ##p < 0.01 vs. H₂O₂ (Analysis of variance (ANOVA), using Dunnett’s post hoc test).