TABLE 2.
CRP is involved in gcvT-lacZ expression
| Straina (plasmid) | Relevant genotypeb | β-Galactosidase activityc of cells grown in GM medium with:
|
||
|---|---|---|---|---|
| No addition | Glycine | Inosine | ||
| GS162 | Wild type | 134 | 854 | 12 |
| GS1068 | Δcrp::Cmr | 32 | 564 | 12 |
| GS1068 (pGS294) | Δcrp::Cmr (vector) | 38 | — | — |
| GS1068 (pGS396) | Δcrp::Cmr (crp+) | 145 | — | — |
| GS1068 (pYZcrp) | Δcrp::Cmr (crp+++) | 118 | — | — |
| GS1068 (p158A) | Δcrp::Cmr (crpAR1+++) | 143 | — | — |
| GS1068 (p162C) | Δcrp::Cmr (crpAR1+++) | 135 | — | — |
| GS1068 (p19A) | Δcrp::Cmr (crpAR2+++) | 236 | — | — |
| GS1068 (p52N) | Δcrp::Cmr (crpAR3+++) | 103 | — | — |
| GS1079 | Δ(cyaA1400)::Knr | 34 | — | — |
| GS1080 | Δ(cyaA1400)::Knr Δcrp::Cmr | 33 | — | — |
a
All strains carry the λgcvT-lacZΔ−341 fusion.
b
crpAR1, crpAR2, and crpAR3, crp with mutant activation domains AR1, AR2, and AR3, respectively. Superscript + and +++ indicate single and multiple copies of the crp allele, respectively.
c
β-Galactosidase activity is in Miller units (25). All standard deviations were within 15% of the means. —, not determined.