TABLE 5.
CRP is dependent on GcvA for regulation of gcvT-lacZ
| Strain | Relevant genotype | β-Galactosidase activitya |
|---|---|---|
| GS162 λgcvT-lacZΔ−341 | Wild type | 154 |
| GS1068 λgcvT-lacZΔ−341 | Δcrp::Cmr | 49 |
| GS1029 λgcvT-lacZΔ−341 | ΔgcvA::Spr | 89 |
| GS1081 λgcvT-lacZΔ−341 | Δcrp::Cmr ΔgcvA::Spr | 105 |
| GS162 λgcvA-lacZ | Wild type | 4.5 |
| GS1068 λgcvA-lacZ | Δcrp::Cmr | 2.0 |
| GS162 λgcvR-lacZ | Wild type | 94 |
| GS1068 λgcvR-lacZ | Δcrp::Cmr | 97 |
Cells were grown in GM medium. β-Galactosidase activity is in Miller units (25). All standard deviations were within 6% of the means.