TABLE 6.
CRP does not indirectly regulate gcvT by controlling gcvA expression
| Strain (plasmid) | Relevant genotype | β-Galactosi-dase activitya |
|---|---|---|
| GS162 λgcvT-lacZΔ−341 | Wild type | 152 |
| GS162 λgcvT-lacZΔ−341 (pGS341) | Wild type (gcvA+) | 183 |
| GS1068 λgcvT-lacZΔ−341 | Δcrp::Cmr | 64 |
| GS1068 λgcvT-lacZΔ−341 (pGS341) | Δcrp::Cmr (gcvA+) | 51 |
| GS1081 λgcvT-lacZΔ−341 | Δcrp::Cmr ΔgcvA::Spr | 97 |
| GS1081 λgcvT-lacZΔ−341 (pGS341) | Δcrp::Cmr ΔgcvA::Spr (gcvA+) | 54 |
| GS1081 λgcvT-lacZΔ−341 (pGS469) | Δcrp::Cmr ΔgcvA::Spr (autoreg-ulatory mutant gcvA+ +15G) | 57 |
| GS162 λgcvA-lacZ +15G | Wild type | 29 |
| GS1068 λgcvA-lacZ +15G | Δcrp::Cmr | 49 |
a
Cells were grown in GM medium. β-Galactosidase activity is in Miller units (25). All standard deviations were within 17% of the means.