Table 1.

List of primers and FASTA accession numbers of the genes analyzed by real time qPCR analysis. All primers were designed in house using NIH’s Primer Blast and acquired from Integrated DNA Technologies (USA).

Gene	Accession number	5ʹ OLIGO	3ʹ OLIGO	Gene Function
CD11b	NM_008401.2	GCAACCATGGAATTCAGCCC	GAGCGGACAGGGTCTAAAGC	Microglia marker
CLDN 1	NM_016674.4	GGCTTCTCTGGGATGGATCG	CTTTGCGAAACGCAGGACAT	Barrier-forming
CLDN 10	NM_001160096.1	CCCAGAATGGGCTACACATA	CCTTCTCCGCCTTGATACTT	Pore-forming
CLDN 15	NM_021719.4	AGGCACACCTTATCTGGCAC	TGCCCCCTGAACAATCACAA	Pore-forming
CLDN 3	NM_009902.4	CCTAGGAACTGTCCAAGCCG	CCCGTTTCATGGTTTGCCTG	Barrier-forming
CLDN 4	NM_009903.2	CGTAGCAACGACAAGCCCTA	TGTCCCCAGCAAGCAGTTAG	Barrier-forming
CLDN 5	NM_013805.4	GTTAAGGCACGGGTAGCACT	TACTTCTGTGACACCGGCAC	Barrier-forming
CLDN 7	NM_016887.6	GCATACTTTCTGGGGGCCA	TGAAGCGACACTCTCACAGC	Pore-forming
CLDN 2	NM_016675.4	CCGTGTTCTGCCAGGATTCTC	AGGAACCAGCGGCGAGTAG	Pore-forming
GABA	NM_019439.3	TGGGAAGGTTTTCCTGACGG	TGAGTGTGGCGTTCGATTCA	Brain neurotransmitter
IL1β	NM_008361.4	GCCACCTTTTGACAGTGATGAG	AGCTTCTCCACAGCCACAAT	Proinflammatory cytokine.
JAM3	NM_023277.4	GCTGTGAGGTCGTTGCTCTA	AGTGGCACATCATTGCGGTA	Barrier-forming
Myo1C	NM_008659.3	CCGATCACCCGAAGAACCAA	CGCCGGAGGTTCTCAATGAA	Scaffolding
OCCLN	XM_011244634.2	CTGACTATGCGGAAAGAGTTGAC	CCAGAGGTGTTGACTTATAGAAAGAC	Barrier-forming
veCad	XM_006530630.1	AATTTGCCCAGCCCTACGAA	ACTGCCCATACTTGACCGTG	Endothelial barrier-forming.
ZO1	NM_009386.2	AAGAAAAAGAATGCACAGAGTTGTT	GAAATCGTGCTGATGTGCCA	Scaffolding
ZO2	NM_001198985.1	AGCTTGTAGTTCTGAGCCGC	CCGACACGGCAATTCCAAAT	Scaffolding
Zonulin	from Sturgeon et al, 2017 (24)	GAATGTGAGGCAGATGACAG	GTGTTCACCCATTGCTTCTC	TJ modulator
18S	X03205	AGAAACGGCTACCACATCCA	CCCTCCAATGGATCCTCGTT	Ribosomal RNA