Figure 3.

LINC00173 positively modulates MGAT1 expression in WT cells.(a) MGAT1 or LINC00173 expression was measured in HFWT and G-401 cells transfected with sh-LINC00173-1/2 or sh-MGAT1-1/2 relative to sh-NC via RT-qPCR. (b) Western blot was performed to examine the protein level of MGAT1 in HFWT and G-401 cells transfected with sh-NC or sh-LINC00173-1/2. (c) The overexpression efficiency of pcDNA3.1-LINC00173 was tested in WT cells through RT-qPCR. (d-e) Dual luciferase reporter experiments were carried out in HEK293T cells with co-transfection of pcDNA3.1-LINC00173 and indicated luciferase reporters. (f) After RNA pull-down assay, RT-qPCR was performed to quantify the enrichment of LINC00173 in the pulldowns of Bio-NC or Bio-MGAT1-3’UTR in HFWT and G-401 cells treated with or without Protease. **P<0.01.