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. 2022 Jun 14;21(17):1795–1810. doi: 10.1080/15384101.2022.2070399

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Figure 6. — MUC3A restrains cell apoptosis and facilitates cell migration in WT.(a) RT-qPCR and western blot were conducted to test knockdown efficiency of sh-MUC3A-1/2/3 and overexpression efficiency of pcDNA3.1-MUC3A in HFWT and G-401 cells. (b-c) Flow cytometry and transwell assays evaluated the effects of MUC3A knockdown on apoptosis and migration of WT cells. (d-e) Flow cytometry and transwell assays were done to determine apoptosis and migration of HFWT and G-401 cells transfected with sh-NC, sh-MGAT1-1, sh-MGAT1-1+pcDNA3.1, or sh-MGAT1-1+pcDNA3.1-MUC3A. **P<0.01.