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12 major classes representing 6 cell lineages of all 3 germ layers were found in the neoblast compartment of adult planaria. Nb2 and SL6a can self-renew, and collectively give rise to a wide range of tissue types in single cell transplantation and repopulation, respectively. As differentiation ensues, piwi-1 expression is downregulated, and tissue-associated transcription factors upregulated.
