FIG. 2.

Northern analysis of sacK, sacB, sacA, and sacR gene expression in L. lactis NZ9800 and NZ9860. Cells were grown to mid-logarithmic phase on either glucose (glu), sucrose (suc), or a mixture of glucose and sucrose (glu/suc), and RNA was isolated, separated on agarose gels, and blotted. Blots were hybridized with DNA probes specific to each of the indicated sac genes. The sizes of the transcripts were estimated by comparing their migration distances to those of RNA markers run in parallel.