Fig 5. Murine model of CDI.

(A) Schematic of mice model. 6-week-old female mice were randomly divided into groups (n = 5 per group). Mice were given antibiotic water consisting of kanamycin (0.4 mg/ml), colistin (850 U/ml), gentamicin (0.035 mg/ml), vancomycin (0.045 mg/ml), and metronidazole (0.215 mg/ml) for 5 days. Clindamycin (10 mg/kg) was injected intraperitoneally a day before infection. C. difficile spores were administered via oral gavage on Day 0. Treatment was initiated on Day 0 and continued for 5 days. A single dose of each drug was administered via oral gavage daily. (B) The Kaplan-Meier survival curve was analyzed using a log-rank (Mantel-Cox) test. Significant difference was noted between the survival curves of the mice treated with NP-003875 vs. untreated control (100% vs. 20%; p = 0.0144).