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. Author manuscript; available in PMC: 2022 Aug 9.
Published in final edited form as: J Vis Exp. 2019 Sep 28;(151):10.3791/60334. doi: 10.3791/60334

Figure 2: Seeding of hESCs onto patterned polyacrylamide hydrogels.

Figure 2:

Representative brightfield images demonstrating successful seeding of hESCs onto polyacrylamide hydrogels with patterned ligand. The timeline at the top indicates the series of media changes used to remove unattached cells and dilute out the Y27632. Note that after initial seeding, cells adhere stochastically to various regions within the patterned ligand and then proliferate to fill the patterned regions over the course of 72 h. Scale bar = 500 μm.