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. 2022 Mar 15;82(6):1013–1024. doi: 10.1158/0008-5472.CAN-21-1707

Figure 2.

Figure 2. DAU causes selective replication stress in MYC-overexpressing cells. A, Immunoblots of the indicated proteins in ARPE-19-MYC (DOX+) and ARPE-19-MYC (DOX−) cells treated with DAU for 24 hours. B, Immunoblots of the indicated proteins in SW480, HCT116, RKO, and Raji cells treated for 24 hours with DAU. C and D, Immunoblot analysis of the effect of MYC expression on DAU-induced Chk1 and ATR phosphorylation. E and F, RKO (E) and HCT116 (F) cells treated with vehicle or DAU (5 μmol/L) in the presence or absence of 50 μmol/L cytidine (cyti), uridine (urid), guanosine (guan), or adenosine (aden) were analyzed by immunoblotting. G and H, HCT116 (G) and RKO (H) cells were pretreated with 200 nmol/L CX-5461 for 12 hours, and then treated with 5 μmol/L DAU for another 5 or 10 hours. Cell extracts were analyzed by immunoblotting. One of three to five similar experiments is shown. DOX, doxycycline; ctrl, control; hr, hours.

DAU causes selective replication stress in MYC-overexpressing cells. A, Immunoblots of the indicated proteins in ARPE-19-MYC (DOX+) and ARPE-19-MYC (DOX−) cells treated with DAU for 24 hours. B, Immunoblots of the indicated proteins in SW480, HCT116, RKO, and Raji cells treated for 24 hours with DAU. C and D, Immunoblot analysis of the effect of MYC expression on DAU-induced Chk1 and ATR phosphorylation. E and F, RKO (E) and HCT116 (F) cells treated with vehicle or DAU (5 μmol/L) in the presence or absence of 50 μmol/L cytidine (cyti), uridine (urid), guanosine (guan), or adenosine (aden) were analyzed by immunoblotting. G and H, HCT116 (G) and RKO (H) cells were pretreated with 200 nmol/L CX-5461 for 12 hours, and then treated with 5 μmol/L DAU for another 5 or 10 hours. Cell extracts were analyzed by immunoblotting. One of three to five similar experiments is shown. DOX, doxycycline; ctrl, control; hr, hours.