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. 2021 Dec 29;82(4):556–570. doi: 10.1158/0008-5472.CAN-21-1446

Figure 2.

Figure 2. Uc003xsl.1 promotes proliferation, migration, and invasion of TNBC cells. A and B, The efficiency of Uc003xsl.1 knockdown in MDA-MB-231-P3 cells (A) and overexpression in BT549 cells (B) was verified by qRT-PCR. C and D, Cell viability after Uc003xsl.1 knockdown in MDA-MB-231-P3 cells (C) and overexpression in BT549 cells (D) was assessed by MTS assays. E–H, Representative images and histogram analysis of transwell assays after Uc003xsl.1 knockdown in MDA-MB-231-P3 cells (E and G) and overexpression in BT549 cells (F and H). Scale bars, 100 μm. I, Gross appearance of xenograft tumors after subcutaneous injections with sh-NC, sh-lnc1, and sh-lnc2 groups (n = 5). J and K, Tumor volumes (J) and weights (K) were measured in the indicated groups (n = 5). L, Representative images of lung colonization and H&E staining of lung sections after injection of MDA-MB-231-P3 cells with sh-NC, sh-lnc1, and sh-lnc2 groups into tail veins of mice (n = 5). Scale bars, 100 μm. M, Histogram analysis for the number of metastatic foci representing lung metastasis (n = 5). Error bars, SDs of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Uc003xsl.1 promotes proliferation, migration, and invasion of TNBC cells. A and B, The efficiency of Uc003xsl.1 knockdown in MDA-MB-231-P3 cells (A) and overexpression in BT549 cells (B) was verified by qRT-PCR. C and D, Cell viability after Uc003xsl.1 knockdown in MDA-MB-231-P3 cells (C) and overexpression in BT549 cells (D) was assessed by MTS assays. E–H, Representative images and histogram analysis of transwell assays after Uc003xsl.1 knockdown in MDA-MB-231-P3 cells (E and G) and overexpression in BT549 cells (F and H). Scale bars, 100 μm. I, Gross appearance of xenograft tumors after subcutaneous injections with sh-NC, sh-lnc1, and sh-lnc2 groups (n = 5). J and K, Tumor volumes (J) and weights (K) were measured in the indicated groups (n = 5). L, Representative images of lung colonization and H&E staining of lung sections after injection of MDA-MB-231-P3 cells with sh-NC, sh-lnc1, and sh-lnc2 groups into tail veins of mice (n = 5). Scale bars, 100 μm. M, Histogram analysis for the number of metastatic foci representing lung metastasis (n = 5). Error bars, SDs of three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.