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. 2021 Dec 29;82(4):556–570. doi: 10.1158/0008-5472.CAN-21-1446

Figure 6.

Figure 6. Synthesis and characterization of siUc003xsl.1/Trop2 NPs. A, Western blotting analysis showed that Trop2 was highly expressed in MDA-MB-231-P3 cells and TNBC cells. B, Immunofluorescence analysis indicated the membrane and cytoplasmic location of Trop2 in MDA-MB-231 cells. C, Schematic illustration of the components of si-Uc003xsl.1/Trop2 NPs: PDSA polymer, lipid-PEG (1,2-distearoyl-sn-glycero-3-phospho-ethanolamine-N-[methoxy(polyethylene glycol)-3000]), DSP E-PEG3k linked with Anti-Trop2 antibody, and cationic lipid G0-C14. D and E, Morphology of the si-Uc003xsl.1 NPs (D) and the si-Uc003xsl.1/Trop2 NPs (E). Scale bars, 100 nm. F and G, Size distribution of si-Uc003xsl.1 NPs (F) and si-Uc003xsl.1/Trop2 NPs (G). H, Uc003xsl.1 expression level detected by qRT-PCR in MDA-MB-231-P3 cells treated with the si-Uc003xsl.1 NPs or si-Uc003xsl.1/Trop2 NPs at different siRNA doses. I and J, Flow cytometry profile (I) and mean fluorescence intensity (MFI; J) of MDA-MB-231-P3 cells incubated with Cy5-labeled naked siRNA, si-Uc003xsl.1 NPs, and si-Uc003xsl.1/Trop2 NPs at 37°C for 4 hours at a 1 nmol/L siRNA dose. K, Blood circulation profile of the naked siRNA, si-Uc003xsl.1 NPs, and si-Uc003xsl.1/Trop2 NPs. L, Overlaid fluorescent image of the nude mice, MDA-MB-231-P3 tumor-bearing mice, and MDA-MB-231-P3-Trop2-KO tumor-bearing mice at 24 hours after injection of the formulas in K. Error bars, SDs of three independent experiments. *, P < 0.05; **, P < 0.01.

Synthesis and characterization of siUc003xsl.1/Trop2 NPs. A, Western blotting analysis showed that Trop2 was highly expressed in MDA-MB-231-P3 cells and TNBC cells. B, Immunofluorescence analysis indicated the membrane and cytoplasmic location of Trop2 in MDA-MB-231 cells. C, Schematic illustration of the components of si-Uc003xsl.1/Trop2 NPs: PDSA polymer, lipid-PEG (1,2-distearoyl-sn-glycero-3-phospho-ethanolamine-N-[methoxy(polyethylene glycol)-3000]), DSP E-PEG3k linked with Anti-Trop2 antibody, and cationic lipid G0-C14. D and E, Morphology of the si-Uc003xsl.1 NPs (D) and the si-Uc003xsl.1/Trop2 NPs (E). Scale bars, 100 nm. F and G, Size distribution of si-Uc003xsl.1 NPs (F) and si-Uc003xsl.1/Trop2 NPs (G). H, Uc003xsl.1 expression level detected by qRT-PCR in MDA-MB-231-P3 cells treated with the si-Uc003xsl.1 NPs or si-Uc003xsl.1/Trop2 NPs at different siRNA doses. I and J, Flow cytometry profile (I) and mean fluorescence intensity (MFI; J) of MDA-MB-231-P3 cells incubated with Cy5-labeled naked siRNA, si-Uc003xsl.1 NPs, and si-Uc003xsl.1/Trop2 NPs at 37°C for 4 hours at a 1 nmol/L siRNA dose. K, Blood circulation profile of the naked siRNA, si-Uc003xsl.1 NPs, and si-Uc003xsl.1/Trop2 NPs. L, Overlaid fluorescent image of the nude mice, MDA-MB-231-P3 tumor-bearing mice, and MDA-MB-231-P3-Trop2-KO tumor-bearing mice at 24 hours after injection of the formulas in K. Error bars, SDs of three independent experiments. *, P < 0.05; **, P < 0.01.