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. 2022 Mar 3;82(5):846–858. doi: 10.1158/0008-5472.CAN-21-2342

Figure 4.

Figure 4. AMPK inhibition with BML-275 before chemotherapy prevents metastasis and increases survival rate by preventing PGCC formation. A, Illustration of the tissue details for nasopharyngeal orthotopic implantation. B, Histology of nasopharyngeal orthotopic tumors. Left, gross observation. Right, hematoxylin and eosin staining for tumor locations (dotted black line). C, Schematic of the detailed treatment strategy. D, Left, hematoxylin and eosin analysis of PGCC number in tumor tissues. Arrow, representative PGCC. Right, quantification of PGCC number (one-way ANOVA). E, IHC analysis of tumor LC3 and P62 expression. F, Quantification of IHC staining (one-way ANOVA). G, Pearson correlation between LC3/P62 expression and PGCC number (linear regression). H, Pattern of NPC metastasis. I, Whole-body BLI was used to track tumor metastasis from the orthotopic site. J and K, Kaplan–Meier analysis was used to compare overall survival (J) and overall metastasis (log-rank test; K). All data represent the mean ± SEM of at least three independent experiments. P values were calculated using one-way ANOVA. ****, P < 0.0001; NS, not significant.

AMPK inhibition with BML-275 before chemotherapy prevents metastasis and increases survival rate by preventing PGCC formation. A, Illustration of the tissue details for nasopharyngeal orthotopic implantation. B, Histology of nasopharyngeal orthotopic tumors. Left, gross observation. Right, hematoxylin and eosin staining for tumor locations (dotted black line). C, Schematic of the detailed treatment strategy. D, Left, hematoxylin and eosin analysis of PGCC number in tumor tissues. Arrow, representative PGCC. Right, quantification of PGCC number (one-way ANOVA). E, IHC analysis of tumor LC3 and P62 expression. F, Quantification of IHC staining (one-way ANOVA). G, Pearson correlation between LC3/P62 expression and PGCC number (linear regression). H, Pattern of NPC metastasis. I, Whole-body BLI was used to track tumor metastasis from the orthotopic site. J and K, Kaplan–Meier analysis was used to compare overall survival (J) and overall metastasis (log-rank test; K). All data represent the mean ± SEM of at least three independent experiments. P values were calculated using one-way ANOVA. ****, P < 0.0001; NS, not significant.