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. 2022 Mar 15;82(10):1937–1952. doi: 10.1158/0008-5472.CAN-21-3038

Figure 6.

Figure 6. PLRG1 occupies both the promoter and superenhancer regions of CCND1 and activates its transcription in liver cancer cells. A, Relative mRNA and protein expression levels of CCND1 upon knockdown of PLRG1 in Huh7 and HepG2-C3A cells (n = 3). B, Relative mRNA and protein expression levels of CCND1 upon overexpression of PLRG1 in Huh7 and HepG2-C3A cells (n = 3). C, Profiles of PLRG1, H3K27ac, and DHX37 occupancy and ATAC-seq peaks at the CCND1 promoter (blue line) and superenhancer (red line) regions in Huh7 cells. Gray shading indicates the two specific constituent superenhancers (SE1 and SE2). D, ChIP-qPCR analysis for PLRG1 enrichment at the promoter and SE2 region of CCND1(n = 3). E, Treatment with siPLRG1 significantly reduced RNA Pol II occupancy at the CCND1 promoter and SE2 region compared with that in the siNC group (n = 3). F, Treatment with siPLRG1 significantly reduced H3K27ac and BRD4 enrichment at the CCND1 SE2 region compared with that in the siNC group (n = 3). The values are expressed as the mean ± SEM (A and B, D–F). ***, P < 0.001; **, P < 0.01; and *, P < 0.05 by one-way ANOVA or two-sided Student t test.

PLRG1 occupies both the promoter and superenhancer regions of CCND1 and activates its transcription in liver cancer cells. A, Relative mRNA and protein expression levels of CCND1 upon knockdown of PLRG1 in Huh7 and HepG2-C3A cells (n = 3). B, Relative mRNA and protein expression levels of CCND1 upon overexpression of PLRG1 in Huh7 and HepG2-C3A cells (n = 3). C, Profiles of PLRG1, H3K27ac, and DHX37 occupancy and ATAC-seq peaks at the CCND1 promoter (blue line) and superenhancer (red line) regions in Huh7 cells. Gray shading indicates the two specific constituent superenhancers (SE1 and SE2). D, ChIP-qPCR analysis for PLRG1 enrichment at the promoter and SE2 region of CCND1 (n = 3). E, Treatment with siPLRG1 significantly reduced RNA Pol II occupancy at the CCND1 promoter and SE2 region compared with that in the siNC group (n = 3). F, Treatment with siPLRG1 significantly reduced H3K27ac and BRD4 enrichment at the CCND1 SE2 region compared with that in the siNC group (n = 3). The values are expressed as the mean ± SEM (A and B, D–F). *, P < 0.05; **, P < 0.01; ***, P < 0.001 by one-way ANOVA or two-sided Student t test.