Table 1.

COX-1, COX-2 and PGE₂ Levels in Different Matrices.

Biological Samples	Source	LPS	Amount of COX-1 (pmol/mg)	Amount of COX-2 (pmol/mg)	Concentration of PGE2 (ng/mL)
Human COX-1 enzyme	Human	—	356.35 ± 25.99	<0.17	—
Human COX-2 enzyme		—	<0.17	3037.68 ± 469.80	—
SCC Patient Specimen #1	Human	—	0.656	<0.056	33.72
SCC Patient Specimen #2		—	1.011	<0.056	25.96
SCC Patient Specimen #3		—	0.736	<0.056	32.06
Human SI microsomes	Human	—	<0.056	<0.056	—
Human colon microsomes		—	<0.056	<0.056	—
SCC9 cells	Human	No	<0.083	<0.083	1.03 ± 0.08
SCC9 cells		Yes	<0.083	0.090 ± 0.009	5.48 ± 0.21
Caco-2	Human	No	<0.083	<0.083	<0.050
Caco-2		Yes	<0.083	<0.083	<0.050
Pirc polyps microsomes	Rat	—	0.84 ± 0.12	0.091 ± 0.075	—
Pirc colon microsomes		—	0.48 ± 0.22	0.023 ± 0.001	—
F344 colon microsomes		—	0.41	<0.014	—
Raw264.7 cells	Mouse	No	<0.042	<0.042	<0.050
Raw264.7 cells		Yes	<0.042	50.89 ± 1.23	12.50 ± 0.61
EOMA cells	Mouse	No	<0.042	4.15 ± 0.04	6.30 ± 0.40
EOMA cells		Yes	0.58 ± 0.10	10.91 ± 0.63	14.30 ± 0.17

Notes: In cell lysate, LLOQ of rodent and human COXs was 0.042pmol/mg and 0.083pmol/mg, respectively. In tissue microsome, LLOQ of rat and human COXs was 0.014pmol/mg and 0.056pmol/mg, respectively. In recombinant enzyme, LLOQ of human COXs was 0.17pmol/mg. LLOQ of PGE₂ in cell culture medium was 0.050 ng/mL.