Fig. 1. Excess individual nucleotides can impair proliferation.
a, Proliferation rates of A549 cells cultured in standard conditions (untreated) or with 1 µM Torin1, without serum (−FBS), without arginine (−Arg), without leucine (−Leu), with 100 nM rotenone, with 5 nM oligomycin, with 1 µM lometrexol (LTX) or with 1 µM brequinar (BRQ). b–f, Proliferation rates of A549 cells treated with the indicated concentration of guanine (G), thymidine (T), deoxyadenosine (dA), cytidine (C) or adenine (A). Each of these nucleobases/nucleosides can be salvaged to produce intracellular nucleotides as shown. Data are presented as mean ± standard deviation (s.d.) of three biological replicates. Source numerical data are available in source data.