Extended Data Fig. 1. Salvage of different nucleobases and nucleosides can inhibit proliferation in different cell types.
a, Intracellular nucleotide levels in A549 cells cultured in standard conditions (Untr.) or treated with 1 µM lometrexol (LTX) or 1 µM brequinar (BRQ) as indicated. b, Proliferation rates of the indicated cells in standard culture conditions (None) or treated with the indicated concentrations of adenine (A), deoxyadenosine (dA), thymidine (T), or guanine (G). Of note, 143B cells are deficient in thymidine kinase, and therefore cannot salvage thymidine to produce dTMP. c, Proliferation rates of RPE-1 cells in standard culture (Untreated) or treated with the indicated concentrations of A, dA, T, G, or cytidine (C). d, Proliferation rates of A549 cells in standard culture conditions (Untreated) or treated with 14 mM deoxycytidine (dC). e, Proliferation rates of A549 cells in standard culture conditions (Untreated) or treated with 200 µM of the indicated nucleobase/nucleoside. f, Normalized proliferation rates of A9 cells that are wild type (WT) or deficient (HPRT-/- APRT-/-) for hypoxanthine-guanine phosphoribosyltransferase and adenine phosphoribosyltransferase in standard culture conditions (Untreated) or treated with 200 µM G or A. Data are presented as mean + /- SD of 3 biological replicates. Source numerical data are available in source data.