TABLE 1.
Primers used to amplify and sequence plsB26
| Primera | Sequence (5′-3′) | bpb |
|---|---|---|
| 1 (+) | TTAGCGCCGCGCGAAACAT | −102 |
| 2 (−) | AACATCACCGACACTGGCA | 386 |
| 3 (+) | AGCTGTTCCACGACTATC | 308 |
| 4 (−) | AGTCGGTTCCAGGTGAAG | 833 |
| 5 (+) | ATGGAAGAGATTGCGGCGAA | 754 |
| 6 (−) | TCGGAATCAGCGTAATCGGA | 1264 |
| 7 (+) | ATTCCGTCGAGTACTTCGTG | 1133 |
| 8 (−) | CACGTTGCGCATCAGATCCA | 1677 |
| 9 (+) | GCATCACGTCAGCGCTCACT | 1600 |
| 10 (−) | CGCATCAATAACGTCCGGCA | 2004 |
| 11 (+) | CCGCGACGTATTGATGGA | 1902 |
| 12 (−) | GTTCTCGAGCCGGCAATTCT | 2450 |
a
Orientation of primer with respect to plsB gene sequence is indicated in parenthesis.
b
Position of 5′ base of primer. A of ATG initiation codon is position 1.