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. 2022 Aug 8;13(8):694. doi: 10.1038/s41419-022-05145-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a The poly(A:T)-mediated IFN antiviral pathway is suppressed in Otud1^−/−-MEFs. Otud1^+/+- and Otud1^−/−-MEF cells were stimulated with 1 μg/ml poly(A:T) for the indicated periods, and the cell lysates were immunoblotted with the indicated antibodies. b The expression of IRF3-target genes is suppressed in poly(A:T)-treated Otud1^−/−-MEF cells. MEFs were stimulated with 1 μg/ml poly(A:T) for the indicated periods, and the mRNA levels were assessed by qPCR. c The suppressed poly(I:C)-mediated antiviral response in Otud1^−/−-MEFs. Otud1^+/+-- and Otud1^−/−-MEFs were stimulated with 10 μg/ml poly(I:C) for the indicated periods, and cell lysates were immunoblotted with the indicated antibodies. d Reduced poly(I:C)-mediated antiviral response in Otud1^−/−-BMDMs. BMDMs from Otud1^+/+-- and Otud1^−/−-mice were stimulated with 10 μg/ml poly(I:C) for the indicated periods, and a qPCR analysis was performed. e, f Sendai virus (SeV)-induced antiviral response is suppressed in Otud1^−/−-cells. Otud1^+/+-- and Otud1^−/−-MEFs (e) or BMDMs (f) were infected with SeV at a CIU of 10 for 1 h, incubated for the indicated periods, and then subjected to qPCR analyses. b, d–f Data are shown as mean ± SD by ANOVA post-hoc Tukey test (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, NS: not significant. g–i Suppressed transcription of IRF3-target genes in Otud1^−/−-MEFs. Otud1^+/+- and Otud1^−/−-MEFs were stimulated with 10 μg/ml poly(I:C) for 2 and 4 h, and RNA-seq analyses were performed. g A principal component analysis of the RNA-seq analysis. h Taking the cutoff value of 10, significantly varied mRNAs are shown by the heatmaps. i Taking the cutoff value of 3.0, pathway analyses of 179 down-regulated genes were performed by MSigDB Hallmark 2020 and listed in ascending order of P-value.