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. 2022 Aug 8;13:4621. doi: 10.1038/s41467-022-32425-7

Fig. 1. MIN6 cells can simultaneously survive chronic ER stress and alter proinsulin proteostasis.

Fig. 1

Western blot analysis (a), protein synthesis, n = 3 (b), and eIF2B GEF activity, n = 3 (c), measured in MIN6 cells treated with CPA as indicated. Cell viability (d), protein synthesis, n = 3 (e), and Western blot analysis (f) measured in MIN6 cells for the indicated treatments. qRT-PCR analysis for Ins1 and Ins2 mRNA levels normalized to GAPDH, n = 3 (g), and Western blot analysis for proinsulin, n = 3 (h), measured in MIN6 cells for the indicated treatments. i Fluorescence immunocytochemistry of proinsulin at 0 and 18 h of CPA treatment and 24 h of washout following CPA treatment. Scale bar represents 50 μm. Two independent experiments; The representative images were shown. Error bars represent S.E.M. p-value represents the statistical test by two-tailed paired Student’s t-test. Representative western blotting images were shown. Dots in all plots represent independent experiments. Source data are provided as a Source Data file.