Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 8;13:4621. doi: 10.1038/s41467-022-32425-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — Fluorescence immunocytochemistry of MIN6 (a), INS1E (d), mouse islets (g) and EndoC βH3 (f), cells treated with CPA for 6 h and 18 h (MIN6), 6 h (INS1E) 18 h (mouse islets) and 72 h (EndoC βH3) for the indicated (color-coded) proteins. Boxes indicated the enlarged regions of images below each panel. b Quantitative analysis of the proinsulin puncta subcellular localization in the indicated treatments of MIN6 cells. c Fluorescence immunocytochemistry of MIN6 cells for the indicated proteins and treatments. e Reconstruction of stacking images represented by the box in d. h Schematic representation of the non-canonical exit of proinsulin from the ER. n, indicates number if cells from 3 independent experiments. The % of cells for the indicated phenotype are given in 2b in colored boxes and columns. Scale bar, 10 μm (a), 20 μm (c, d, f), 50 μm (g).