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. 2022 Aug 8;13:4621. doi: 10.1038/s41467-022-32425-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Scatterplots of fold changes in CPA1 vs CON (a) and CPA18 vs CON (b). c, h Scatterplot of fold changes of the UPR signature (GO:0030968) gene expression between acute (CPA1/CON) and chronic (CPA18/CON) ER stress. d Scatterplot of fold changes of genes in groups identified in Fig. 3E (G6 and G3) in acute (CPA1/CON) and chronic (CPA18/CON) ER stress. e, i, j qRT-PCR analysis for the indicated mRNAs normalized to GAPDH mRNA levels, in MIN6 cells treated with CPA for the indicated times, n = 3. f, l Western blot analysis for the indicated proteins in MIN6 cells. g Scatterplot representing an association between Ins2 and MafA mRNA levels measured by qRT-PCR. k qRT-PCR analysis of Pcsk2 mRNA levels normalized to GAPDH mRNA levels, in MIN6 cells under the indicated treatments, n = 3. m Western blot analysis of proinsulin in non-reducing SDS-PAGE electrophoresis. Error bars represent S.E.M. p-value represents the statistical test by two-tailed paired Student’s t-test. Representative western blotting images were shown. Dots in all plots represent independent experiments. Source data are provided as a Source Data file.