Table 3.
Statistics for microarray and qRT-PCR analyses.
| Gene | Two-way ANOVA results | Post hoc comparison (Tukey’s test) results (p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) |
|---|---|---|
| Gadd45b (M, microarray) | Sig interaction of genotype & treatment F (1, 12) = 18.39, p = 0.0011 | WT vs WT ECS ***, WT ECS vs KO ECS *** |
| Gadd45b (M, qRT PCR) | Sig interaction of genotype & treatment F (1, 12) = 12.55, p = 0.0041 | WT vs WT ECS ***, WT ECS vs KO ECS ** |
| Gadd45b (F, qRT PCR) |
Sig effect of genotype F (1, 14) = 5.302, p = 0.0372; Sig effect of treatment F (1, 14) = 15.80, p = 0.0014 |
WT vs WT ECS **, WT ECS vs KO ECS * |
| Gadd45g (M, microarray) | Sig interaction of genotype & treatment F (1, 12) = 18.39, p = 0.0011 | WT vs WT ECS ***, WT ECS vs KO ECS ** |
| Gadd45g (M, qRT PCR) | Sig interaction of genotype & treatment F (1, 12) = 9.875, p = 0.0085 | WT vs WT ECS ***, WT ECS vs KO ECS * |
| Gadd45g (F, qRT PCR) | Not sig | N/A |
| Cdnk1a (M, microarray) | Sig interaction of genotype & treatment F (1, 12) = 7.316, p = 0.0191 | WT vs WT ECS ****, KO vs KO ECS *, WT ECS vs KO ECS * |
| Cenpa (M, microarray) | Sig interaction of genotype & treatment F (1, 12) = 263.1, p < 0.0001 | WT vs WT ECS ****, WT ECS vs KO ECS **** |
| Cenpa (M, qRT PCR) | Sig interaction of genotype & treatment F (1, 12) = 103.9, p < 0.0001 | WT vs WT ECS ****, WT ECS vs KO ECS **** |
| Cenpa (F, qRT PCR) | Sig interaction of genotype & treatment F (1, 14) = 68.28, p < 0.0001 | WT vs WT ECS ****, WT ECS vs KO ECS **** |
| Fos (M, microarray) | Sig interaction of genotype & treatment F (1, 12) = 10.92, p = 0.0063 | WT vs WT ECS ****, KO vs KO ECS *, WT ECS vs KO ECS** |
| Fos (M, qRT PCR) | Sig interaction of genotype & treatment F (1, 12) = 5.808, p = 0.0329 | WT vs WT ECS **, WT ECS vs KO ECS * |
| Fos (F, qRT PCR) | Sig effect of treatment F (1, 14) = 8.392, p = 0.0117 | WT vs WT ECS * |
| Fosb (M, microarray) | Sig interaction of genotype & treatment F (1, 12) = 63.79, p < 0.0001 | WT vs WT ECS ****, WT ECS vs KO ECS **** |
| Fosb (M, qRT PCR) | Sig interaction of genotype & treatment F (1, 12) = 14.01, p = 0.0028 | WT vs WT ECS ***, WT ECS vs KO ECS *** |
| Fosb (F, qRT PCR) | Sig interaction of genotype & treatment F (1, 14) = 7.094, p = 0.0185 | WT vs WT ECS ***, WT ECS vs KO ECS ** |
| Junb (M, microarray) | Sig interaction of genotype & treatment F (1, 12) = 18.22, p = 0.0011 | WT vs WT ECS ****, KO vs KO ECS *, WT ECS vs KO ECS*** |
| Junb (M, qRT PCR) | Sig effect of treatment F (1, 12) = 6.247, p = 0.0280 | Posthoc comparisons NS |
| Junb (F, qRT PCR) |
Sig effect of treatment F (1, 14) = 6.569, p = 0.0225; sig effect of genotype F (1, 14) = 4.608, p = 0.0498 |
Posthoc comparisons NS |
| Nr4a2 (M, microarray) | Sig interaction of genotype & treatment F (1, 12) = 15.90, p = 0.0018 | WT vs WT ECS ***, WT ECS vs KO ECS *** |
| Nr4a2 (M, qRT PCR) | Sig interaction of genotype & treatment F (1, 12) = 7.050, p = 0.0210 | WT vs WT ECS ***, WT ECS vs KO ECS ** |
| Nr4a2 (F, qRT PCR) | Sig interaction of genotype & treatment F (1, 14) = 5.230, p = 0.0383 | WT vs WT ECS * |
| Mef2c (M, microarray) | Sig interaction of genotype & treatment F (1, 12) = 21.07, p = 0.0006 | WT vs WT ECS**, KO vs. WT ECS** |
| Mef2c (M, qRT PCR) | Sig interaction of genotype & treatment F (1, 12) = 9.484, p = 0.0095 | Posthoc comparisons NS |
| Mef2c (F, qRT PCR) | Not sig | N/A |
| Calb2 (M, microarray) | Sig effect of genotype F (1, 12) = 48.99, p < 0.0001 | WT vs KO***, WT ECS vs. KO ECS** |
| Calb2 (M, qRT PCR) | Sig effect of genotype F (1, 12) = 11, p = 0.0061 | Posthoc comparisons NS |
| Calb2 (F, qRT PCR) | Not sig | N/A |